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anti histone h2b (Cell Signaling Technology Inc)

Name: anti histone h2b
Brand: Cell Signaling Technology Inc
Rating: 94 (60 reviews)

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Cell Signaling Technology Inc anti histone h2b
$(A) Nuclear, cytosolic, and mitochondrial fractions of LNCaP cells were subjected to RT-qPCR for the quantification of U6 snRNA, mitochondrial 12S rRNA, and 18S rRNA, as well as to TaqMan RT-qPCR for the quantification of 5′-tRNA halves. U6 snRNA, mitochondrial 12S rRNA, and 18S rRNA served as controls for nuclear, mitochondrial, and cytosolic RNA, respectively. (B) Proteins interacting with 5′-tRNA LysCUU half from pull-down experiments were developed by SDS–PAGE. Three prominent bands were subjected to mass-spec protein identification. The identified Top 5 proteins are shown. (C) The total protein lysate (Whole), nuclear, and cytosolic fractions of LNCaP cells were subjected to western blots. <t>H2B</t> and GAPDH were used as nuclear and cytosolic protein controls, respectively. (D) Cytosolic lysates from pull-down experiments using biotinylated control RNA or 5′-/3′-tRNA LysCUU half were subjected to western blot for YBX1. Input lysates before pull-down experiments were also analyzed as a control. (E, G) Total RNAs extracted from YBX1 KD (E) or OE (G) cells were subjected to RT-qPCR/TaqMan RT-qPCR for quantification of YBX1 mRNA, p21 mRNA, and 5′-tRNA LysCUU half. For all bar graphs in the present study, error bars indicate mean ± SD of triplicate measurements (* P < 0.05, ** P < 0.01, and *** P < 0.001; two-tailed t -Test). (F) Lysates from the YBX1 OE cells were subjected to western blots. (H) The relative abundance of the cells after transfection of the indicated siRNAs. To ensure consistency, the total amounts of siRNA were adjusted to be identical. The condition transfected only with control siRNA contained double the amount of the control siRNA, as the other two conditions contained two species of siRNAs. Abundance on the day of transfection was set at 1. Raw images of B, C, D, and F are located in S1 Raw images. The data underlying the graphs can be found in .$
Anti Histone H2b, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti histone h2b/product/Cell Signaling Technology Inc
Average 94 stars, based on 60 article reviews

anti histone h2b - by Bioz Stars, 2026-02

94/100 stars

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1) Product Images from "A hormone-dependent tRNA half promotes cell cycle progression via destabilization of p21 mRNA"

Article Title: A hormone-dependent tRNA half promotes cell cycle progression via destabilization of p21 mRNA

Journal: PLOS Biology

doi: 10.1371/journal.pbio.3003194

$(A) Nuclear, cytosolic, and mitochondrial fractions of LNCaP cells were subjected to RT-qPCR for the quantification of U6 snRNA, mitochondrial 12S rRNA, and 18S rRNA, as well as to TaqMan RT-qPCR for the quantification of 5′-tRNA halves. U6 snRNA, mitochondrial 12S rRNA, and 18S rRNA served as controls for nuclear, mitochondrial, and cytosolic RNA, respectively. (B) Proteins interacting with 5′-tRNA LysCUU half from pull-down experiments were developed by SDS–PAGE. Three prominent bands were subjected to mass-spec protein identification. The identified Top 5 proteins are shown. (C) The total protein lysate (Whole), nuclear, and cytosolic fractions of LNCaP cells were subjected to western blots. H2B and GAPDH were used as nuclear and cytosolic protein controls, respectively. (D) Cytosolic lysates from pull-down experiments using biotinylated control RNA or 5′-/3′-tRNA LysCUU half were subjected to western blot for YBX1. Input lysates before pull-down experiments were also analyzed as a control. (E, G) Total RNAs extracted from YBX1 KD (E) or OE (G) cells were subjected to RT-qPCR/TaqMan RT-qPCR for quantification of YBX1 mRNA, p21 mRNA, and 5′-tRNA LysCUU half. For all bar graphs in the present study, error bars indicate mean ± SD of triplicate measurements (* P < 0.05, ** P < 0.01, and *** P < 0.001; two-tailed t -Test). (F) Lysates from the YBX1 OE cells were subjected to western blots. (H) The relative abundance of the cells after transfection of the indicated siRNAs. To ensure consistency, the total amounts of siRNA were adjusted to be identical. The condition transfected only with control siRNA contained double the amount of the control siRNA, as the other two conditions contained two species of siRNAs. Abundance on the day of transfection was set at 1. Raw images of B, C, D, and F are located in S1 Raw images. The data underlying the graphs can be found in .$
Figure Legend Snippet: (A) Nuclear, cytosolic, and mitochondrial fractions of LNCaP cells were subjected to RT-qPCR for the quantification of U6 snRNA, mitochondrial 12S rRNA, and 18S rRNA, as well as to TaqMan RT-qPCR for the quantification of 5′-tRNA halves. U6 snRNA, mitochondrial 12S rRNA, and 18S rRNA served as controls for nuclear, mitochondrial, and cytosolic RNA, respectively. (B) Proteins interacting with 5′-tRNA LysCUU half from pull-down experiments were developed by SDS–PAGE. Three prominent bands were subjected to mass-spec protein identification. The identified Top 5 proteins are shown. (C) The total protein lysate (Whole), nuclear, and cytosolic fractions of LNCaP cells were subjected to western blots. H2B and GAPDH were used as nuclear and cytosolic protein controls, respectively. (D) Cytosolic lysates from pull-down experiments using biotinylated control RNA or 5′-/3′-tRNA LysCUU half were subjected to western blot for YBX1. Input lysates before pull-down experiments were also analyzed as a control. (E, G) Total RNAs extracted from YBX1 KD (E) or OE (G) cells were subjected to RT-qPCR/TaqMan RT-qPCR for quantification of YBX1 mRNA, p21 mRNA, and 5′-tRNA LysCUU half. For all bar graphs in the present study, error bars indicate mean ± SD of triplicate measurements (* P < 0.05, ** P < 0.01, and *** P < 0.001; two-tailed t -Test). (F) Lysates from the YBX1 OE cells were subjected to western blots. (H) The relative abundance of the cells after transfection of the indicated siRNAs. To ensure consistency, the total amounts of siRNA were adjusted to be identical. The condition transfected only with control siRNA contained double the amount of the control siRNA, as the other two conditions contained two species of siRNAs. Abundance on the day of transfection was set at 1. Raw images of B, C, D, and F are located in S1 Raw images. The data underlying the graphs can be found in .

Techniques Used: Quantitative RT-PCR, SDS Page, Mass Spectrometry, Western Blot, Control, Two Tailed Test, Transfection

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Journal: PLOS Biology

Article Title: A hormone-dependent tRNA half promotes cell cycle progression via destabilization of p21 mRNA

doi: 10.1371/journal.pbio.3003194

Figure Lengend Snippet: (A) Nuclear, cytosolic, and mitochondrial fractions of LNCaP cells were subjected to RT-qPCR for the quantification of U6 snRNA, mitochondrial 12S rRNA, and 18S rRNA, as well as to TaqMan RT-qPCR for the quantification of 5′-tRNA halves. U6 snRNA, mitochondrial 12S rRNA, and 18S rRNA served as controls for nuclear, mitochondrial, and cytosolic RNA, respectively. (B) Proteins interacting with 5′-tRNA LysCUU half from pull-down experiments were developed by SDS–PAGE. Three prominent bands were subjected to mass-spec protein identification. The identified Top 5 proteins are shown. (C) The total protein lysate (Whole), nuclear, and cytosolic fractions of LNCaP cells were subjected to western blots. H2B and GAPDH were used as nuclear and cytosolic protein controls, respectively. (D) Cytosolic lysates from pull-down experiments using biotinylated control RNA or 5′-/3′-tRNA LysCUU half were subjected to western blot for YBX1. Input lysates before pull-down experiments were also analyzed as a control. (E, G) Total RNAs extracted from YBX1 KD (E) or OE (G) cells were subjected to RT-qPCR/TaqMan RT-qPCR for quantification of YBX1 mRNA, p21 mRNA, and 5′-tRNA LysCUU half. For all bar graphs in the present study, error bars indicate mean ± SD of triplicate measurements (* P < 0.05, ** P < 0.01, and *** P < 0.001; two-tailed t -Test). (F) Lysates from the YBX1 OE cells were subjected to western blots. (H) The relative abundance of the cells after transfection of the indicated siRNAs. To ensure consistency, the total amounts of siRNA were adjusted to be identical. The condition transfected only with control siRNA contained double the amount of the control siRNA, as the other two conditions contained two species of siRNAs. Abundance on the day of transfection was set at 1. Raw images of B, C, D, and F are located in S1 Raw images. The data underlying the graphs can be found in .

Article Snippet: The following antibodies were used: anti-p21 (Sigma–Aldrich, OP64), anti-GAPDH (Cell signaling, 2,118), anti-YB1 (Abcam, ab12148), anti-Flag (Sigma–Aldrich, F3165), anti-Histone H2B (Cell signaling, 8135S), and anti-β-Tubulin (Developmental Studies Hybridoma Bank, E7).

Techniques: Quantitative RT-PCR, SDS Page, Mass Spectrometry, Western Blot, Control, Two Tailed Test, Transfection